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plasmid encoding erα  (Addgene inc)


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    Structured Review

    Addgene inc plasmid encoding erα
    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and <t>ERα</t> (red dots) in MCF-7 cells and <t>transfected</t> <t>HEK293T</t> cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.
    Plasmid Encoding Erα, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+encoding+er%CE%B1/pEGFP-C1-ER+alpha+(Plasmid+%2328230)/bio_rxiv__64898__2026__03__17__712321-363-9-14
    Average 93 stars, based on 47 article reviews
    plasmid encoding erα - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Antidepressants interact with sex steroid receptors and their intracellular signaling components"

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components

    Journal: bioRxiv

    doi: 10.64898/2026.03.17.712321

    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.
    Figure Legend Snippet: In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Techniques Used: In Situ, Transfection, Expressing, Staining

    Related Articles

    Transfection:

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components
    Article Snippet: .. A group of HEK293T cells were transfected with a plasmid encoding ERα (pEGFP-C1-ER alpha, Addgene, Plasmid #28230) using Lipofectamine 2000 (Thermo Fisher Scientific). ..

    Plasmid Preparation:

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components
    Article Snippet: .. A group of HEK293T cells were transfected with a plasmid encoding ERα (pEGFP-C1-ER alpha, Addgene, Plasmid #28230) using Lipofectamine 2000 (Thermo Fisher Scientific). ..



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    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and <t>ERα</t> (red dots) in MCF-7 cells and <t>transfected</t> <t>HEK293T</t> cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.
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    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and <t>ERα</t> (red dots) in MCF-7 cells and <t>transfected</t> <t>HEK293T</t> cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.
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    Image Search Results


    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Journal: bioRxiv

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components

    doi: 10.64898/2026.03.17.712321

    Figure Lengend Snippet: In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Article Snippet: A group of HEK293T cells were transfected with a plasmid encoding ERα (pEGFP-C1-ER alpha, Addgene, Plasmid #28230) using Lipofectamine 2000 (Thermo Fisher Scientific).

    Techniques: In Situ, Transfection, Expressing, Staining